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PA0552 Rev B
Page 2
Intended Use
This reagent is for in vitro diagnostic use.
Pax-5 (1EW) monoclonal antibody is intended to be used for the qualitative identication by light microscopy of human Pax-5 in formalin-
xed, parafn-embedded tissue by immunohistochemical staining using the automated Bond
TM
system.
The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and
should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualied pathologist.
Summary and Explanation
Immunohistochemical techniques can be used to demonstrate the presence of antigens in tissue and cells (see “Using Bond Reagents”
in your Bond user documentation). Pax-5 (1EW) primary antibody is a ready to use product that has been specically optimized for use
with Bond Polymer Rene Detection. The demonstration of human Pax-5 is achieved by rst, allowing the binding of Pax-5 (1EW) to the
section, and then visualizing this binding using the reagents provided in the detection system. The use of these products, in combination
with the automated Bond system, reduces the possibility of human error and inherent variability resulting from individual reagent dilution,
manual pipetting and reagent application.
Reagents Provided
Pax-5 (1EW) is a mouse anti-human monoclonal antibody produced as a tissue culture supernatant, and supplied in Tris buffered saline
with carrier protein, containing 0.35% ProClin
TM
950 as a preservative.
Total volume = 7 mL.
Clone
1EW.
Immunogen
Recombinant protein corresponding to part of the C-terminus of the human Pax-5 molecule.
Specicity
Human Pax-5.
Subclass
IgG1.
Total Protein Concentration
Approx 10 mg/mL.
Antibody Concentration
Greater than or equal to 0.34 mg/L as determined by ELISA.
Dilution and Mixing
Pax-5 (1EW) primary antibody is optimally diluted for use on the Bond system. Reconstitution, mixing, dilution or titration of this reagent
is not required.
Materials Required But Not Provided
Refer to “Using Bond Reagents” in your Bond user documentation for a complete list of materials required for specimen treatment and
immunohistochemical staining using the Bond system.
Storage and Stability
Store at 2–8 °C. Do not use after the expiration date indicated on the container label.
The signs indicating contamination and/or instability of Pax-5 (1EW) are: turbidity of the solution, odor development, and presence of
precipitate.
Return to 2–8 °C immediately after use.
Storage conditions other than those specied above must be veried by the user
1
.
Precautions
• This product is intended for in vitro diagnostic use.
• The concentration of ProClin
™
950 is 0.35%. It contains the active ingredient 2-methyl-4-isothiazolin-3-one, and may cause irritation to
the skin, eyes, mucous membranes and upper respiratory tract. Wear disposable gloves when handling reagents.
• To obtain a copy of the Material Safety Data Sheet contact your local distributor or regional ofce of Leica Biosystems, or
alternatively, visit the Leica Biosystems’ Web site, www.LeicaBiosystems.com.
• Specimens, before and after xation, and all materials exposed to them, should be handled as if capable of transmitting infection and
disposed of with proper precautions
2
. Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with
reagents or specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water. Seek
medical advice.
Bond
™
Ready-To-Use Primary Antibody
Pax-5 (1EW)
Catalog No: PA0552